Analysis of E. coli K5 capsular polysaccharide heparosan
نویسندگان
چکیده
منابع مشابه
Analysis of E. coli K5 capsular polysaccharide heparosan.
Heparosan is the key precursor for the preparation of bioengineered heparin, a potential replacement for porcine intestinal heparin, an important anticoagulant drug. The molecular weight (MW) distribution of heparosan produced by the fermentation of E. coli K5 was investigated. Large-slab isocratic and mini-slab gradient polyacrylamide gel electrophoresis (PAGE) were used to analyze the MW and ...
متن کاملBiosynthesis of the Escherichia coli K5 capsular polysaccharide.
299-304 47 Payne, G., Virji, M. and Saunden, J. R ( I 996) in Abstracts of the Tenth International Pathogenic Neisseria Conference (Zollinger, W.D., Frasch, C.E. and Deal, C.D., eds.), pp. 393-394, National Institutes of Health, Bethesda, MD 48 Parge, H. E.. Forest, K T.. Hickey, M. 1.. Christensen, D. A,. Getzoff, E. D. and Tainer, J. A. (I 995) Nature (London) 378, 32-38 49 Forest, K T. and T...
متن کاملNuclear magnetic resonance quantification for monitoring heparosan K5 capsular polysaccharide production.
Traditional chromatographic quantification methods for heparosan produced from the Escherichia coli K5 strain rely on extensive purification requiring laborious sample preparation. These methods are time-consuming, often resulting in sample loss during purification, and thus might not accurately reflect the amount of heparosan in the original mixture. A simple, sensitive (1)H nuclear magnetic r...
متن کاملE. coli K5 fermentation and the preparation of heparosan, a bioengineered heparin precursor.
Heparosan is an acidic polysaccharide natural product, which serves as the critical precursor in heparin biosynthesis and in the chemoenzymatic synthesis of bioengineered heparin. Heparosan is also the capsular polysaccharide of Escherichia coli K5 strain. The current study was focused on the examination of the fermentation of E. coli K5 with the goal of producing heparosan in high yield and vo...
متن کاملEscherichia coli K5 heparosan fermentation and improvement by genetic engineering.
N-acetyl heparosan is the precursor for the biosynthesis of the important anticoagulant drug heparin. The E. coli K5 capsular heparosan polysaccharide provides a promising precursor for in vitro chemoenzymatic production of bioengineered heparin. This article explores the improvements of heparosan production for bioengineered heparin by fermentation process engineering and genetic engineering.
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ژورنال
عنوان ژورنال: Analytical and Bioanalytical Chemistry
سال: 2010
ISSN: 1618-2642,1618-2650
DOI: 10.1007/s00216-010-3679-7